Additional Selective and Differential Cell Culture Media
BrainBits Hibernate A, 100 mL
Hibernate is a nutrient medium for the maintenance of neural tissue or cells in ambient carbon dioxide levels.
Promocell GmbH PromoCell Endothelial Cell Growth Medium MV 2 500 ml, ready-to-use, low serum, with C-39226 attached to the flask
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Follicle Dermal Papilla Cell Growth Medium KIT 500 ml, Contains: C-26500, C-39620
PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells.The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of Follicle Dermal Papilla Cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.04 ml / ml || Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Melanocyte Basal Medium 500 ml
PromoCell Melanocyte Growth Medium is a PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M3.While the supplements of the Melanocyte Growth Medium are serum-free, the Basal Medium is manufactured with 0.2% serum.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Phorbol Myristate Acetate | 10 ng / ml |
STEMCELL Technologies StemSpan™ Leukemic Cell Culture Kit, Size: 1 Kit
StemSpan™ Leukemic Cell Culture Kit has been developed for the in vitro culture and expansion of malignant cells and has been tested on chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) samples. This optimized protocol allows users to expand, culture, and use malignant cells for drug screening. StemSpan™ Leukemic Cell Culture Kit includes the serum-free medium SFEM II (Catalog #09605), StemSpan™ CD34+ Expansion Supplement (10X; Catalog #02691), and small molecule UM729 (Catalog #72332).
Lonza Walkersville HepaRG Preinduction and Toxicity Medium Supplement
HepaRG Pre-induction and Toxicity Medium Supplement with corticoids, to be combined with 100 mL of Basal medium (Catalog #MH100) w/o phenol red. Completed Pre-induction/Tox Medium is most commonly used for toxicity assays using the NoSpin HepaRG cells.Benefits:Transitional medium for No Spin HepaRG cells just prior to toxicity or induction applicationsApplications:ADMEToxContent:1 conical tube with 12.5mL supplement
Bioworld Potato Dextrose Broth, 500 g
Potato Dextrose BrothUsed for the cultivation of yeasts and molds especially Fusarium speciesTo inhibit bacterial growth, the medium may be acidified to pH 3.5 by adding tartaric acid, lactic acid or some other organic acid The medium must not be heated after the addition of the acid, this will result in the hydrolysis of the agar and destroy its gelling propertiesPrepared Appearance: light amber brothSoluble in boiling waterComposition:Potato Infusion Glucose
Bioworld Murashige and Skoog(MS) Medium, Modification No. 4, 25 L
Murashige & Skoog (MS) MediumContains Macronutrients and Micronutrients with No NH4NO3 as described by Murashige & Skoog (1962)Sucrose, Cytokines, Vitamins, and Auxins should be added as neededProvided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking concentration 2.7 g/L
Promocell GmbH PromoCell Myocyte Growth Medium 500 ml, ready-to-use, low serum, with Supplement Mix C-39275 attached to the flask
The PromoCell Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine and primary rat cardiac myocytes.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 0.5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 2 ng / ml || Insulin (recombinant human) | 5 µg / ml |
Promocell GmbH PromoCell Airway Epithelial Cell Basal Medium (prf) 500 ml, phenol red-free
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Promocell GmbH PromoCell Preadipocyte Basal Medium 500 ml
PromoCell Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Hydrocortisone | 1 μg / ml || Heparin | 90 μg / ml |
Promocell GmbH PromoCell Chondrocyte Growth Medium 500 ml, ready-to-use, low-serum, with C-39635 attached to the flask
PromoCell Chondrocyte Growth Medium has been developed for the in vitro cultivation of human chondrocytes. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for primary porcine and rat chondrocytes.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.1 ml / ml |
Promocell GmbH PromoCell Endothelial Cell Growth Medium 2 500 ml, ready-to-use, low serum, with Supplement Mix C-39216 attached to the flask
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its multiple effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Biotang Inc TBS/TWEEN-20 (10X) 500 ML, IBB-580, 1% Tween-20 in 250 mM Tris-HCl, 27 mM KCl and 1.37 M NaCl, pH 7.4 +/- 0.2. Prepared in 18.2 megohm water.and autoclaved at 15 psi for 30 min on liquid cycle.
TBS/TWEEN-20 (10X) 500 ML, IBB-580, 1% Tween-20 in 250 mM Tris-HCl, 27 mM KCl and 1.37 M NaCl, pH 7.4 +/- 0.2. Prepared in 18.2 megohm water.and autoclaved at 15 psi for 30 min on liquid cycle.